Quantification of Substrate Reducing Sugars in Pineapple Residues Using the 3,5-Dinitrosalicylic Acid Method
Abstract
Pineapple waste has a large number of sugars, which can be used by microorganisms to degrade them and generate metabolites or fermentation products. Related works apply the method proposed by Miller, which uses 3,5-dinitrosalicylic acid to determine the concentration of reducing sugars. In this exercise, the Miller’s method was employed to determine reducing sugars of the substrate in pineapple (Ananas cosmosus) presented in a fermentation broth with Lactobacillus acidophillus using an experimental method that adds 3,5-dinitrosalicylic acid or DNS to each sample. It is a colorimetric method to determine light absorbances at a wavelength of 540 nm. The DNS reactant was initially prepared, a glucose calibration curve was prepared with different concentrations. Samples were taken from the fermented broth. 3,5-dinitrosalicylic acid was added to each glucose solution and each sample. Besides, the absorbances of each solution were determined in a spectrophotometer. A correlation coefficient equal to 0,993 was obtained in the calibration curve. The values of the substrate concentrations were reached in a range between 2,773 g/L to 4,654 g/L. A correlation coefficient close to 1 was found, which indicates the perfect correlation between data. Also, as the fermentation time increased, the concentration of reducing sugars decreased. Miller’s method showed a high correlation coefficient, being an effective method for the determination of reducing sugars in pineapple.
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